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Fundamental concepts relevant to many engineering disciplines, including: energy, vectors, force systems, free body diagrams, strength of materials, associated problem-solving, and basic design procedures.
Architextures (ARTX), is a library of high quality seamless textures for use in architectural drawings and 3D models. All textures on the site are procedural meaning the dimensions, patterns, colours and more can be edited using Architextures Create, our custom-built web app for creating seamless textures. All textures can be downloaded free of charge for educational and personal use. Textures can be used in commercial work by users with a Pro subscription subject to the Terms of Use. By using the Architextures website you agree to the comply with these Terms of Use.
Chain reactions, characterized by initiation, propagation and termination, are stochastic at microscopic scales and underlie vital chemical (for example, combustion engines), nuclear and biotechnological (for example, polymerase chain reaction) applications1,2,3,4,5. At macroscopic scales, chain reactions are deterministic and limited to applications for entertainment and art such as falling dominoes and Rube Goldberg machines. On the other hand, the microfluidic lab-on-a-chip (also called a micro-total analysis system)6,7 was visualized as an integrated chip, akin to microelectronic integrated circuits, yet in practice remains dependent on cumbersome peripherals, connections and a computer for automation8,9,10,11. Capillary microfluidics integrate energy supply and flow control onto a single chip by using capillary phenomena, but programmability remains rudimentary with at most a handful (eight) operations possible12,13,14,15,16,17,18,19. Here we introduce the microfluidic chain reaction (MCR) as the conditional, structurally programmed propagation of capillary flow events. Monolithic chips integrating a MCR are three-dimensionally printed, and powered by the free energy of a paper pump, autonomously execute liquid handling algorithms step-by-step. With MCR, we automated (1) the sequential release of 300 aliquots across chained, interconnected chips, (2) a protocol for severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) antibodies detection in saliva and (3) a thrombin generation assay by continuous subsampling and analysis of coagulation-activated plasma with parallel operations including timers, iterative cycles of synchronous flow and stop-flow operations. MCRs are untethered from and unencumbered by peripherals, encode programs structurally in situ and can form a frugal, versatile, bona fide lab-on-a-chip with wide-ranging applications in liquid handling and point-of-care diagnostics.
MCRs require ancillary capillary microfluidic components that fulfil different functions depending on the intended operation (for example, loading, holding, mixing and draining liquids following the MCR progression) to form fully integrated and scalable capillaric circuits (CCs). CCs are designed on the basis of a library of building blocks including capillary pumps, flow resistances and many types of capillary valve (stop valves (SVs), trigger valves, retention valves, retention burst valves (RBVs))12,14, and thus are analogous to microelectronic integrated circuits, but lacking the scalability and functionality. In MCRs, samples are loaded by capillary flow through an inlet with a capillary retention valve and entirely fill the reservoirs lined with three SVs, including two with a dual RBV function connecting to the two lateral CDVs, and one at the intersection of the functional connection and the main channel (Fig. 1c). Although the functional connection is a deceptively simple straight channel, it fulfils six key functions. It is (1) the air vent during filling of the reservoir, and (2) a SV preventing the reagent from spilling into the main channel while it is empty. After filling of the main channel, it forms a (3) hydraulic link propagating the pressure from the main channel into the reservoir and (4) a barrier (and bottleneck) to the diffusion of reagents between the reservoir and the main channel. (5) It becomes the outlet and a flow resistance (discussed further below) during reservoir emptying, and (6) a capillary retention valve stopping air from invading the main conduit after the reservoir is emptied. As a result, many trade-offs guide its design.
In accordance with the art. 13 section 1 and 2 of the European Parliament and Council Regulation 2016/679 of the 27th April, 2016 on the protection of natural persons, with regard to the processing of personal data and on the free movement of such data, and repealing Directive 95/46/EC (General Data Protection Regulation), hereafter RODO, I hereby inform that: 2ff7e9595c
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